Cultures of human embryonic stem cells: serum replacement medium or serum-containing media and the effect of basic fibroblast growth factor.

@article{Koivisto2004CulturesOH,
  title={Cultures of human embryonic stem cells: serum replacement medium or serum-containing media and the effect of basic fibroblast growth factor.},
  author={Heidi Koivisto and Marjukka Hyv{\"a}rinen and A Str{\"o}mberg and Jos{\'e} Antonio Inzunza and Eija Matilainen and Milla Mikkola and Outi Hovatta and Heli Teerijoki},
  journal={Reproductive biomedicine online},
  year={2004},
  volume={9 3},
  pages={
          330-7
        }
}
Human embryonic stem (hES) cells have traditionally been cultured in medium containing fetal calf serum (FCS) and mouse fibroblasts as feeder cells. The use of animal derived materials carries a risk of transmitting animal pathogens, and they are not optimal in cultures aimed at cell transplantation in humans. This technical study aiming at facilitating IVF units to establish new hES cell lines, has systematically compared the non-differentiated growth of the hES cell line HS237, originally… CONTINUE READING

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