Cultured bovine endothelial cells produce both urokinase and tissue- type plasminogen activators

  title={Cultured bovine endothelial cells produce both urokinase and tissue- type plasminogen activators},
  author={Eugene G. Levin and David J. Loskutoff},
  journal={The Journal of Cell Biology},
  pages={631 - 636}
Cell extracts and conditioned media (CM) from cultured bovine aortic endothelial cells (BAEs) were fractionated by PAGE in the presence SDS, and plasminogen activator (PA) activity was localized by fibrin autography. Multiple molecular weight forms of PA were detected in both preparations. Cell-associated PAs had Mr of 48,000, 74,000, and 100,000 while secreted PAs showed Mr of 52,000, 74,000, and 100,000. A broad zone of activity (Mr 80,000-100,000) also was present in both cellular fractions… CONTINUE READING


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