Cultured bovine endothelial cells produce both urokinase and tissue- type plasminogen activators

@article{Levin1982CulturedBE,
  title={Cultured bovine endothelial cells produce both urokinase and tissue- type plasminogen activators},
  author={Eugene G. Levin and David J. Loskutoff},
  journal={The Journal of Cell Biology},
  year={1982},
  volume={94},
  pages={631 - 636}
}
Cell extracts and conditioned media (CM) from cultured bovine aortic endothelial cells (BAEs) were fractionated by PAGE in the presence SDS, and plasminogen activator (PA) activity was localized by fibrin autography. Multiple molecular weight forms of PA were detected in both preparations. Cell-associated PAs had Mr of 48,000, 74,000, and 100,000 while secreted PAs showed Mr of 52,000, 74,000, and 100,000. A broad zone of activity (Mr 80,000-100,000) also was present in both cellular fractions… CONTINUE READING

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References

Publications referenced by this paper.
Showing 1-10 of 19 references

Clonal endothelial cell growth , Fed

  • W. Laug
  • 1982

Purification and characterization of the plasminogen activator secreted by human melanoma cells in culture

  • J. K. Christman, S. C. Silverstein
  • J . BioL Chem .
  • 1981

On the regulation and control of flbrinolysis, Thromb. Haemoslasis

  • D. Collen
  • 1980
2 Excerpts

Comparative studies of the firbinolytic activity o f cultured vascular ceils

  • U. K. Laemmli
  • Thromb . Res .
  • 1979

Immunological characterization of multiple molecular weight forms of human cell plasminogen activators

  • E. G. Hoal, E. B. Dowdle
  • J . BioZ Chem .
  • 1979

A study of protease and protease - inhibitor complexes in biological fluids

  • M. M. Bradford
  • J . Exp . Med .
  • 1978

Immunological analysis of plasminogen activators from normal and transformed hamster cells

  • D. Vetterlein, T. E Bell P. L. Young
  • J . Exp . Med .
  • 1978

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