Culture system for rodent and human oligodendrocyte specification, lineage progression, and maturation.

Abstract

Here we document protocols for the production, isolation, and maintenance of the oligodendrocyte phenotype from rodent and human neural stem cells. Our unique method relies on a series of chemically defined media, specifically designed and carefully characterized for each developmental stage of oligodendrocytes as they advance from oligodendrocyte progenitors to mature, myelinating oligodendrocytes.

DOI: 10.1002/9780470151808.sc02d04s10

Cite this paper

@article{EspinosaJeffrey2009CultureSF, title={Culture system for rodent and human oligodendrocyte specification, lineage progression, and maturation.}, author={Araceli Espinosa-Jeffrey and Dustin R. Wakeman and Byung Seok Kim and Evan Yale Snyder and Jean de Vellis}, journal={Current protocols in stem cell biology}, year={2009}, volume={Chapter 2}, pages={Unit 2D.4} }