Culture scale-up studies as seen from the viewpoint of oxygen supply and dissolved carbon dioxide stripping.

@article{Matsunaga2009CultureSS,
  title={Culture scale-up studies as seen from the viewpoint of oxygen supply and dissolved carbon dioxide stripping.},
  author={Naoki Matsunaga and Kenjiro Kano and Yasuyuki Maki and Toshiaki Dobashi},
  journal={Journal of bioscience and bioengineering},
  year={2009},
  volume={107 4},
  pages={412-8}
}
Oxygen supply and dissolved carbon dioxide (dCO(2)) stripping are two of the most important control parameters in cell culture. In this study, we investigated the effect of scale-up on the volumetric gas transfer coefficient with bioreactors of different sizes (working volume: 80 L, 500 L, 2000 L, and 10,000 L; aspect ratio: 1.0-1.6). Sparging air into water increased the volumetric oxygen transfer coefficient (k(L)a), an index of oxygen supply efficiency, by scale-up roughly in proportion to… CONTINUE READING
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