Culture of reconstructed epidermis in a defined medium at 33° C shows a delayed epidermal maturation, prolonged lifespan and improved stratum corneum

@article{Gibbs1997CultureOR,
  title={Culture of reconstructed epidermis in a defined medium at 33° C shows a delayed epidermal maturation, prolonged lifespan and improved stratum corneum},
  author={Susan Gibbs and Jana Vi{\vc}anov{\'a} and Joke A Bouwstra and Dirk Valstar and Johanna Kempenaar and Maria Ponec},
  journal={Archives of Dermatological Research},
  year={1997},
  volume={289},
  pages={585-595}
}
In this study we compared human keratinocyte cultures grown at the air-liquid interface on de-epidermized dermis at 33° C or at 37° C in two different culture media: medium I – a fully defined serum- and EGF-free medium; and medium II – a serum- and EGF-containing medium. Cultures grown in medium II were initially hyperproliferative followed rapidly by senescence, and had a high triglyceride content. The hyperproliferation was ascribed to the presence of EGF in the medium. In contrast, cultures… CONTINUE READING

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