Culture of previously uncultured members of the human gut microbiota by culturomics

  title={Culture of previously uncultured members of the human gut microbiota by culturomics},
  author={Jean-Christophe Lagier and Saber Khelaifia and Maryam Tidjani Alou and Sokhna Ndongo and Niokhor Dione and P. Leroux Hugon and Aur{\'e}lia Caputo and Fr{\'e}d{\'e}ric Cadoret and Sory Ibrahima Traore and El Hadji Seck and Gr{\'e}gory Dubourg and Guillaume Durand and Ga{\"e}l Mourembou and Elodie Guilhot and Amadou Hamidou Togo and Sara Bellali and Dipankar Bachar and Nadim Cassir and Fadi Bittar and J{\'e}r{\'e}my Delerce and Morgane Mailhe and Davide Ricaboni and Melhem Bilen and Nicole Prisca Makaya Dangui Nieko and Ndeye M{\'e}ry Dia Badiane and Camille Valles and Donia Mouelhi and Khoudia Diop and Matthieu Million and Didier Musso and J{\^o}natas Santos Abrah{\~a}o and Esam I Azhar and Fehmida Bibi and Muhammad Yasir and Aldiouma Diallo and Cheikh Sokhna and Felix Djossou and V Vitton and Catherine Robert and Jean Marc Rolain and Bernard La Scola and P Fournier and Anthony Levasseur and Didier Raoult},
  journal={Nature Microbiology},
Metagenomics revolutionized the understanding of the relations among the human microbiome, health and diseases, but generated a countless number of sequences that have not been assigned to a known microorganism1. The pure culture of prokaryotes, neglected in recent decades, remains essential to elucidating the role of these organisms2. We recently introduced microbial culturomics, a culturing approach that uses multiple culture conditions and matrix-assisted laser desorption/ionization–time of… CONTINUE READING
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