Culture of pea embryo axes for studies on the reactivation of the cell cycle at germination

@article{Levi1992CultureOP,
  title={Culture of pea embryo axes for studies on the reactivation of the cell cycle at germination},
  author={Marisa Levi and Elena Pasini and Paola Brusa and Donato Chiatante and Sergio Sgorbati and Elio Sparvoli},
  journal={In Vitro Cellular & Developmental Biology - Plant},
  year={1992},
  volume={28},
  pages={20-24}
}
SummaryA method for culturing excised pea embryo axes, which allows growth of large batches of embryo axes in a reduced volume of medium and is suitable for performing biochemical analyses and early treatment or labeling of embryos with exogenous substances, has been devised. Pea embryo axes were excised from the seeds after 3 h of imbibition and cultured in a mineral medium containing sucrose, vitamins, and casamino acids. Under these conditions, the reactivation of the cell cycle occurs much… CONTINUE READING

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