Culture of in vitro produced bovine zygotes in vitro vs in vivo: implications for early embryo development and quality.

@article{Enright2000CultureOI,
  title={Culture of in vitro produced bovine zygotes in vitro vs in vivo: implications for early embryo development and quality.},
  author={Brian P Enright and Patrick Lonergan and Andr{\'a}s Dinny{\'e}s and T. Keith Fair and Fabian Ward and Xiaona Yang and Maurice P. Boland},
  journal={Theriogenology},
  year={2000},
  volume={54 5},
  pages={
          659-73
        }
}
The objectives of this study were to examine the effect of culture system on bovine blastocyst formation rates and quality. Presumptive IVM/IVF bovine zygotes were cultured either in vitro in synthetic oviduct fluid (SOF, 25 embryos/25 microL in 5% CO2, 5% O2, 90% N2 at 39 degrees C) or in vivo in the ewe oviduct (approximately 100 embryos per oviduct). The recovery rate after in vivo culture was 53% (813/1,530). The blastocyst rate on Day 7 was significantly higher for the in vitro system (28… CONTINUE READING
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