Culture of embryonic-like stem cells from human umbilical cord blood and onward differentiation to neural cells in vitro

@article{McGuckin2008CultureOE,
  title={Culture of embryonic-like stem cells from human umbilical cord blood and onward differentiation to neural cells in vitro},
  author={Colin P. McGuckin and Marcin Jurga and Hamad Ali and Marko Strbad and Nicolas Forraz},
  journal={Nature Protocols},
  year={2008},
  volume={3},
  pages={1046-1055}
}
This 3-week protocol produces embryonic-like stem cells from human umbilical cord blood (CBEs) for neural differentiation using a three-step system (cell isolation/expansion/differentiation). The CBE isolation produces a highly purified fraction (CD45−, CD33−, CD7−, CD235a−) of small pluripotent stem cells (2–3 μm in diameter) coexpressing embryonic stem cell markers including Oct4 and Sox2. Initial CBE expansion is performed in high density (5–10 millions per ml) in the presence of… CONTINUE READING
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