Culture of embryonic C. elegans cells for electrophysiological and pharmacological analyses.

  title={Culture of embryonic C. elegans cells for electrophysiological and pharmacological analyses.},
  author={Laura Bianchi and Monica Driscoll},
  journal={WormBook : the online review of C. elegans biology},
Despite the considerable advantages that C. elegans offers for studying gene function in vivo, this system is quite challenging for in vivo electrophysiological analysis of channel function, particularly in neurons. A major problem is that C. elegans neurons are confined in a pressurized and hard-to-penetrate cuticle. Recently, a method for culturing C. elegans embryonic cells has been developed and numerous researchers have already applied this option to study a variety of native ion channels… CONTINUE READING
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