Culture of bovine embryos in buffalo rat liver cell-conditioned media or with leukemia inhibitory factor.

@article{Funston1997CultureOB,
  title={Culture of bovine embryos in buffalo rat liver cell-conditioned media or with leukemia inhibitory factor.},
  author={Richard N. Funston and Wytze J. Nauta and George E. Seidel},
  journal={Journal of animal science},
  year={1997},
  volume={75 5},
  pages={1332-6}
}
Experiments were designed to study development of bovine embryos in TCM-199 medium conditioned by preculture with buffalo rat liver (BRL) cells. Conditioned media were harvested after BRL cells were cultured until confluency (CON), or for an additional 2 d with the same cells but new medium (CON-N) or the same medium (CON-S). Glucose in TCM-199 was depleted by BRL cells to different concentrations depending on coculture procedures: CON = 3.94 mM, CON-N = 1.67 mM, and CON-S = 1.11 mM glucose. In… CONTINUE READING
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References

Publications referenced by this paper.
Showing 1-10 of 19 references

Effect of human leukemia inhibiting factor on in vitro development of IVF - derived bovine morulae and blastocysts

  • E. S. Lee, T. Mogoe, K. K. Lee, Y. Fukui
  • 1995

Current status of IVM / IVF and embryo culture in humans and farm animals

  • A. Trounson, D. Pushett, L. J. Maclellan, I. Lewis, D. K. Gardner
  • Theriogenology
  • 1994
2 Excerpts

Effect of human leukemia inhibitory factor on in vitro development of parthenogenetic bovine morulae

  • T. Saito, A. Miyamoto, H. Yamashina, Y. Okamoto
  • 1994

Supplementation of bovine embryo culture media with FSH , BSA and / or superoxide dismutase

  • S. E. Olson, G. E. Seidel
  • Biol . Reprod .
  • 1994
1 Excerpt

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