Culture of Immature Embryos of Coconut , Cocos nucifera L : Callus Proliferation and Somatic Embryogenesis

  title={Culture of Immature Embryos of Coconut , Cocos nucifera L : Callus Proliferation and Somatic Embryogenesis},
  author={Seetha Karunaratne and KAUSHALYA PERIYAPPERUMA},
The culture of immature embryos of coconut (Cocos nucifera L var. typica) was attempted with a view to developing a technique for clonal propagation. Embryos, 6-7 months postanthesis produced whitish, compact, embryogenic callus tissues in the presence of 12-20 uM 2,4-D. Callus was maintained by subculture on a medium of the same composition. About 50% of the callus cultures produced globular embryos when transferred to 8 pM 2,4-D. Embryos in 22% of these cultures germinated and produced shoots… CONTINUE READING


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