Culture medium pH is influenced by basal medium, carbohydrate source, gelling agent, activated charcoal, and medium storage method

@article{Owen1991CultureMP,
  title={Culture medium pH is influenced by basal medium, carbohydrate source, gelling agent, activated charcoal, and medium storage method},
  author={Henry Owen and Donna Wengerd and al. R. Miller},
  journal={Plant Cell Reports},
  year={1991},
  volume={10},
  pages={583-586}
}
When four carbohydrates were tested against six commonly cited inorganic basal media, post-autoclave pH was highest for carbohydrate-free and sucrose containing media, and progressively lower for maltoseglucose and fructose-containing media, respectively. Post-autoclave pH for these media without carbohydrates was related to medium buffering capacity. Addition of gelling agents (10 of 11 tested) increased the postautoclave pH of MS medium containing sucrose. Neutralized and acid-washed… CONTINUE READING
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