Culture-independent phylogenetic analysis of the faecal flora of the rat.

  title={Culture-independent phylogenetic analysis of the faecal flora of the rat.},
  author={Stephen P. J. Brooks and Murdoch K. McAllister and M Sandoz and Martin L. Kalmokoff},
  journal={Canadian journal of microbiology},
  volume={49 10},
The dominant faecal flora of the rat was determined using randomly cloned 16S rDNA comparative sequence analysis. A total of 109 near full-length 16S rDNA clones were sequenced, representing 69 unique 16S rRNA phylotypes or operational taxonomic units (OTUs). Estimates of species richness indicated that approximately 338 species were present in the faeces, suggesting that only 20% of species were identified. Only two of 39 Gram-negative clones aligned with previously cultured species, the… CONTINUE READING

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