Culture-independent analysis of vaginal microflora: the unrecognized association of Atopobium vaginae with bacterial vaginosis.

@article{Verstraelen2004CultureindependentAO,
  title={Culture-independent analysis of vaginal microflora: the unrecognized association of Atopobium vaginae with bacterial vaginosis.},
  author={Hans Verstraelen and Rita Verhelst and Geert Claeys and Marleen Temmerman and Mario Vaneechoutte},
  journal={American journal of obstetrics and gynecology},
  year={2004},
  volume={191 4},
  pages={1130-2}
}
Although the pathogenesis of bacterial vaginosis remains elusive, a few microorganisms, such as Gardnerella vaginalis, are considered markers on Gram stain or culture. Culture-independent analysis of vaginal microflora using 16S rDNA cloning and sequencing of total bacterial communities reveals the gram-positive Atopobium vaginae as a predominant species in disturbed vaginal flora. 

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