Culture and adenoviral infection of adult mouse cardiac myocytes: methods for cellular genetic physiology.

@article{Zhou2000CultureAA,
  title={Culture and adenoviral infection of adult mouse cardiac myocytes: methods for cellular genetic physiology.},
  author={Yu Ying Zhou and Shi-Qiang Wang and W Z Zhu and Andrzej J. Chruscinski and Brian K. Kobilka and Bruce D. Ziman and Edward G. Lakatta and Heping Cheng and Rui Ping Xiao},
  journal={American journal of physiology. Heart and circulatory physiology},
  year={2000},
  volume={279 1},
  pages={H429-36}
}
Rapid development of transgenic and gene-targeted mice and acute genetic manipulation via gene transfer vector systems have provided powerful tools for cardiovascular research. To facilitate the phenotyping of genetically engineered murine models at the cellular and subcellular levels and to implement acute gene transfer techniques in single mouse cardiomyocytes, we have modified and improved current enzymatic methods to isolate a high yield of high-quality adult mouse myocytes (5.3 +/- 0.5 x… CONTINUE READING
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