Culture-Dependent and -Independent Methods Capture Different Microbial Community Fractions in Hydrocarbon-Contaminated Soils

@inproceedings{Stefani2015CultureDependentA,
  title={Culture-Dependent and -Independent Methods Capture Different Microbial Community Fractions in Hydrocarbon-Contaminated Soils},
  author={Franck O. P. Stefani and Terrence H Bell and Charlotte Marchand and Ivan Enrique de la Providencia and Abdel El Yassimi and Marc St-Arnaud and Mohamed Hijri and Shuijin Hu},
  booktitle={PloS one},
  year={2015}
}
Bioremediation is a cost-effective and sustainable approach for treating polluted soils, but our ability to improve on current bioremediation strategies depends on our ability to isolate microorganisms from these soils. Although culturing is widely used in bioremediation research and applications, it is unknown whether the composition of cultured isolates closely mirrors the indigenous microbial community from contaminated soils. To assess this, we paired culture-independent (454-pyrosequencing… CONTINUE READING
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