Cultural condition affecting the growth and production of â-galactosidase by lactobacillus acidophilus NRRL 4495

  title={Cultural condition affecting the growth and production of {\^a}-galactosidase by lactobacillus acidophilus NRRL 4495},
  author={Siham Abdel-Azeem Ismail and Yasser El-Mohamady and Wafaa H A Helmy and Rasha Abou-Romia and Amal Mohamed Hashem},
The production of â-galactosidase by Lactobacillus acidophilus NRRL 4495 was studied. The highest yield of intracellular â-galactosidase was achieved using acid whey (3.5%) as carbon source after 48h fermentation. Ammonium sulphate (3%) was the best nitrogen source. Addition of 2 MnCl at 0.02M optimized the production of the enzyme. In addition, the activity reached 51.46U/g cells when the temperature of the fermentation optimized at 40°C. The highest â-galactosidase activity was obtained by… CONTINUE READING


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