Cultivation of the rhabditid Poikilolaimus oxycercus as a laboratory nematode for genetic analyses.

  title={Cultivation of the rhabditid Poikilolaimus oxycercus as a laboratory nematode for genetic analyses.},
  author={Ray L. Hong and Andreas Villwock and Ralf J. Sommer},
  journal={Journal of experimental zoology. Part A, Comparative experimental biology},
  volume={303 9},
Vulva formation is a paradigm for evolutionary developmental biology in nematodes. Not only do the number of vulval precursor cells (VPCs) differ between members in the Rhabditidae and Diplogastridae, they are also sculpted via different developmental mechanisms, either by cell fusion in most Rhabditidae or by programmed cell death in the Diplogastridae. In this context, the species Poikilolaimus oxycercus is the only known species in the family Rhabditidae to have a subset of the Pn.p cells… 

Figures and Tables from this paper

First report of Caenorhabditis brenneri (Nematoda: Rhabditida) isolated from the cadaver of Philippinella moellendorffi (Stylommatophora: Ariophantidae), a terrestrial slug in the Philippines

This is the first record of C. brenneri isolated from the terrestrial slug P. moellendorffi, strain IZSP from the Philippines, and the newly isolated nematode was identified as Caenorhabditis b Brenneri.

First report, morphological and molecular characterization of Caenorhabditis brenneri (Nematoda: Rhabditidae) isolated from the giant African land snail Achatina fulica (Gastropoda: Achatinidae)

Nematodes found inside a dissected A. fulica cadaver from Kabacan, North Cotabato and Mindanao, Philippines were identified as Caenorhabditis brenneri, the first report of C. b Brenneri-gastropod association, to be reported for the first time.

S0022149X18000305jra 319..331

The terrestrial gastropod parasite Phasmarhabditis hermaphrodita is proposed as a new alternative to study the evolution of parasitism, and it is shown that this genetically amenable parasite is amenable to forward genetics and that unc and sma mutants can be generated using formaldehyde mutagenesis.

Genome Architecture and Evolution of a Unichromosomal Asexual Nematode

Pristionchus pacificus: a well-rounded nematode.

  • Ray L. HongR. Sommer
  • Biology
    BioEssays : news and reviews in molecular, cellular and developmental biology
  • 2006
This work has been capitalizing on the whole genome sequence of P. pacificus to describe more thoroughly the molecular basis for changes in development, behavior and ecology in nematode evolution, and to better integrate the molecular knowledge with the biodiversity of Pristionchus species.

Description of Abursanema iranicum n. gen., n. sp. (Nematoda: Hexatylina, Sphaerularioidea) from Iran and its phylogenetic relationships.

The new genus Abursanema iranicum is characterized by its smooth outer and annulated inner cuticle, having two incisures in lateral field, lacking stylet knobs, having pyriform terminal bulb with stem-like extension projecting into the intestine and lacking of bursa in male.

A novel copro-diagnostic molecular method for qualitative detection and identification of parasitic nematodes in amphibians and reptiles

A non-invasive PCR-based methodology for sensitive detection and identification of parasitic nematode DNA released in the faeces of infected amphibians as egg or tissue fragments (environmental DNA), which mitigates problems associated with microscopic identification and can be applied to detect nematodes parasitoses in wild and captive hosts for infection surveillance and maintenance of healthy populations.

Eukaryotic opportunists dominate the deep-subsurface biosphere in South Africa

The discovery of Protozoa, Fungi, Platyhelminthes, Rotifera, Annelida and Arthropoda from 1.4 km below ground is reported, and calculations suggest that food, not dissolved oxygen is the limiting factor for eukaryal population growth.

A phylogenetic test of the Red Queen Hypothesis: Outcrossing and parasitism in the Nematode phylum

It is found that selfing and asexuality are significantly less likely to arise on parasitic lineages than on free‐living ones, and this finding is consistent with the Red Queen Hypothesis.



Microevolutionary analysis of the nematode genus Pristionchus suggests a recent evolution of redundant developmental mechanisms during vulva formation

The molecular and developmental characterization of laboratory strains of the nematode genus Pristionchus are described, which lays a foundation for a microevolutionary analysis of vulva development.

Evolution of vulva development in the Cephalobina (Nematoda).

The first example of a vulval cell lineage that is asymmetric between the anterior and the posterior sides of the vulva is described, and it is concluded that in this group, death of the Pn.p cells probably constitutes a derived character state compared to a syncytial fate.

Evolution of cell lineage and pattern formation in the vulval equivalence group of rhabditid nematodes.

This work has analyzed cell lineages and pattern formation in the vulva equivalence group of six rhabditid nematodes of the genera Oscheius, Rhabditella, R Habditoides, Pelodera, and Protorhabditis, and reveals evolutionary modification at several levels.

Comparative developmental studies using Oscheius/Dolichorhabditis sp. CEW1 (Rhabditidae).

This hermaphroditic species can be cultured and handled like C. elegans and is amenable to genetic analysis and the isolation of morphological markers is discussed.

The Nematode Caenorhabditis elegans

This "Book of the Worm" serves as a reference source for C. elegans investigators as well as an introductory monograph for other biologists.

18S ribosomal RNA gene phylogeny for some Rhabditidae related to Caenorhabditis.

18S rDNA sequences cannot be used to resolve relationships between taxa as closely related as the Caenorhabditis species, and Parsimony, minimum-evolution, and maximum-likelihood methods strongly reject Andrássy's proposed phylogenetic classification based on adult morphological characters but support that of Sudhaus as one of a few possible phylogenies.

Defining wild-type life span in Caenorhabditis elegans.

  • D. GemsD. Riddle
  • Biology
    The journals of gerontology. Series A, Biological sciences and medical sciences
  • 2000
It is inferred that the longest-lived N2 variant best resembles the original N2 isolate, which is the N2 male stock currently distributed by the Caenorhabditis Genetics Center.

Description of Caenorhabditis japonica n. sp. (Nematoda: Rhabditida) associated with the burrower bug Parastrachia japonensis (Heteroptera: Cydnidae) in Japan

The species is closely related to species of the Caenorhabditis elegans group and shares many characters with them, but differs from these species in having blunt spicule tips of complex shape and in lacking a terminal notch in the bursa velum.