Cultivation of rat granulosa cells in a serum-free chemically defined medium--a useful model to study lipoprotein metabolism.

@article{Azhar1988CultivationOR,
  title={Cultivation of rat granulosa cells in a serum-free chemically defined medium--a useful model to study lipoprotein metabolism.},
  author={Salman Azhar and Li-Jen Tsai and W Maffe and Eve P. Reaven},
  journal={Biochimica et biophysica acta},
  year={1988},
  volume={963 2},
  pages={
          139-50
        }
}
We have developed a chemically defined, serum-free medium for the culture of rat granulosa cells. This medium contains Dulbecco's modified Eagle's medium/Ham's nutrient F12 (DME:F12) (1:1) plus insulin (2 micrograms/ml), hydrocortisone (100 ng/ml), transferrin (5 micrograms/ml) and fibronectin (2 micrograms/cm2). Granulosa cells grown in this medium have an absolute requirement for added cholesterol-rich lipoproteins for steroidogenesis. When cells are cultured in basal medium, progestin… CONTINUE READING
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