Cultivation of methanogens under low-hydrogen conditions by using the coculture method.

@article{Sakai2009CultivationOM,
  title={Cultivation of methanogens under low-hydrogen conditions by using the coculture method.},
  author={Sanae Sakai and Hiroyuki Imachi and Yuji Sekiguchi and I-Cheng Tseng and Akiyoshi Ohashi and Hideki Harada and Yoichi Kamagata},
  journal={Applied and environmental microbiology},
  year={2009},
  volume={75 14},
  pages={4892-6}
}
We previously reported the isolation of novel methanogens by using a new cultivation method, referred to as the coculture method. Here, we extended our coculture method to various anaerobic environmental samples. As a result, we successfully cultivated some uncharacterized methanogens in coculture enrichments and eventually isolated a new methanogen, within the order Methanomicrobiales. 
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