Cultivation of attenuated hepatitis A virus antigen in a titanium static mixer reactor.

@article{Junker1994CultivationOA,
  title={Cultivation of attenuated hepatitis A virus antigen in a titanium static mixer reactor.},
  author={Beth Helene Junker and T C Seamans and K Ramasubramanyan and John G. Aunins and Etienne Paul and Barry C. Buckland},
  journal={Biotechnology and bioengineering},
  year={1994},
  volume={44 11},
  pages={1315-24}
}
The titanium static mixer reactor, demonstrated for a variety of vaccine processes during the late 197s, was investigated for the production of attenuated hepatitis A virus antigen from anchorage-dependent MRC-5 cells. This reactor system used Charles River Biotechnological Services cabinets for monitoring and process control. Cell inoculation protocols, using 6000-10,000 cells/cm(2), resulted on over 95% attachment at both the laboratory and pilot scales. Indirect monitoring techniques using… CONTINUE READING