Cultivation of an immortalized human corneal endothelial cell population and two distinct clonal subpopulations on thermo-responsive carriers

@article{Gtze2008CultivationOA,
  title={Cultivation of an immortalized human corneal endothelial cell population and two distinct clonal subpopulations on thermo-responsive carriers},
  author={Thomas G{\"o}tze and Monika Valtink and Mirko Nitschke and Stefan Gramm and Thomas Hanke and Katrin Engelmann and Carsten Werner},
  journal={Graefe's Archive for Clinical and Experimental Ophthalmology},
  year={2008},
  volume={246},
  pages={1575-1583}
}
Recently, it was possible to show that human corneal endothelial cells (HCEC) can be cultured on thermo-responsive polymer substrates, and can be harvested as entire cell sheets without losing viability. We sought to study HCEC sheet cultivation on such cell culture carriers under serum-free conditions as the next consequential step in developing methods for generation of corneal endothelial cell transplants. An immortalized heterogenous HCEC population and two immortalized, clonally grown HCEC… CONTINUE READING

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References

Publications referenced by this paper.
Showing 1-10 of 32 references

Detachment of human endothelial cell sheets from thermo-responsive poly (NiPAAm-co-DEGMA) carriers

M Nitschke, T Götze, S Gramm, C Werner
Express Polym Lett 1:660–666, • 2007
View 1 Excerpt

Functional biomedical polymers for corneal regenerative medicine

JY Lai, GH Hsiue
Reactive Funct Polymers 67:1284– • 2007
View 1 Excerpt

Functional copolymers of N-isopropylacrylamide for bioengineering applications

ZMO Rzaev, S Dincer, E Piskin
Prog Polym Sci 32:534–595, • 2007
View 1 Excerpt

Low pressure plasma based approaches to fluorocarbon polymer surface modification

M Nitschke, U König, +3 authors S Zschoche
J Appl Polym Sci • 2007
View 1 Excerpt

Temperature-responsive cell culture surfaces for regenerative medicine with cell sheet engineering

M Yamato, Y Akiyama, +3 authors T Okano
Prog Polym Sci 32:1123–1133, • 2007
View 1 Excerpt

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