Cultivation of V79 cells in serum-free medium containing bovine serum albumin and the use of gelatin as an attachment factor.

@article{Umeda1983CultivationOV,
  title={Cultivation of V79 cells in serum-free medium containing bovine serum albumin and the use of gelatin as an attachment factor.},
  author={Makoto Umeda and Kazuki Noda and Kaori Tanaka and Isao Yamane and Yutaka Matsuya},
  journal={The Tohoku journal of experimental medicine},
  year={1983},
  volume={141 1},
  pages={99-106}
}
V79 cells were cultivated in a serum-free medium. When a serum-free, bovine serum albumin-containing medium was used, removal of trypsin after cell detachment during subcultivation was necessary for routine mass cultivation. For colony formation addition of an attachment factor to the medium was further necessary. Gelatin was proved to serve as a good… CONTINUE READING