Cultivation of Schistosoma mansoni in vitro. I. Establishment of cultures from cercariae and development until pairing.

@article{Basch1981CultivationOS,
  title={Cultivation of Schistosoma mansoni in vitro. I. Establishment of cultures from cercariae and development until pairing.},
  author={Paul F. Basch},
  journal={The Journal of parasitology},
  year={1981},
  volume={67 2},
  pages={179-85}
}
Procedures are described for mass cultivation of Schistosoma mansoni. Cercariae were pooled, concentrated, and axenized through a series of washes in base medium containing antibiotics. Cercariae were sheared through a double-Luer-ended needle connected to two syringes, and tails separated and discarded. Young schistosomules were grown in a medium based upon BME augmented with lactalbumin hydrolysate, glucose, hormones, and other amendments and supplemented with human serum. Human blood cells… CONTINUE READING

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