Cultivation-independent and -dependent characterization of Bacteria resident beneath John Evans Glacier.

  title={Cultivation-independent and -dependent characterization of Bacteria resident beneath John Evans Glacier.},
  author={Stephanie M Cheng and Julia M. Foght},
  journal={FEMS microbiology ecology},
  volume={59 2},
Viable microorganisms are present in subglacial waters and sediment-laden ice beneath John Evans glacier in the Canadian high Arctic. The Bacterial communities resident in three subglacial samples were examined by amplifying 16S rRNA genes extracted from community DNA and from axenic isolates. Restriction fragment length polymorphism analysis of 341 clones produced 153 operational taxonomic units (OTUS), of which 25 dominant OTUS were sequenced. A subglacial water sample yielded… CONTINUE READING
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