Cultivation and growth characteristics of a diverse group of oligotrophic marine Gammaproteobacteria.

@article{Cho2004CultivationAG,
  title={Cultivation and growth characteristics of a diverse group of oligotrophic marine Gammaproteobacteria.},
  author={Jang-Cheon Cho and Stephen J. Giovannoni},
  journal={Applied and environmental microbiology},
  year={2004},
  volume={70 1},
  pages={432-40}
}
Forty-four novel strains of Gammaproteobacteria were cultivated from coastal and pelagic regions of the Pacific Ocean using high-throughput culturing methods that rely on dilution to extinction in very low nutrient media. Phylogenetic analysis showed that the isolates fell into five rRNA clades, all of which contained rRNA gene sequences reported previously from seawater environmental gene clone libraries (SAR92, OM60, OM182, BD1-7, and KI89A). Bootstrap analyses of phylogenetic reliability did… CONTINUE READING

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