Cultivation and characterisation of avian lymphocytes with natural killer cell activity.

@article{Schat1986CultivationAC,
  title={Cultivation and characterisation of avian lymphocytes with natural killer cell activity.},
  author={Karel Antoni Schat and B. W. Calnek and Daniel Weinstock},
  journal={Avian pathology : journal of the W.V.P.A},
  year={1986},
  volume={15 3},
  pages={539-56}
}
Conditioned medium (CM), as a presumed source of lymphokines including interleukin-2, was prepared from chicken spleen cell cultures stimulated with concanavalin A (con A). When CM was used to cultivate spleen cells from 6- to 8-week-old P-2 chickens, eight of nine spleens yielded cell lines which grew continuously for at least 50 days. Six of the cultures were tested for natural killer (NK) cell activity against LSCC-RP9, a lymphoblastoid cell known to be susceptible to NK cells, and against… CONTINUE READING