Cultivation and Laboratory Maintenance of Chlamydia trachomatis.

  title={Cultivation and Laboratory Maintenance of Chlamydia trachomatis.},
  author={Marci A. Scidmore},
  journal={Current protocols in microbiology},
  volume={Chapter 11},
  pages={Unit 11A.1}
Chlamydia species are obligate intracellular bacteria that require growth inside mammalian cells for propagation and survival. As a result, Chlamydia cannot be grown on conventional bacteriological medium. This property makes Chlamydia difficult organisms to grow and maintain in the laboratory. Up until 1965, passage in the yolk sac of the embryonated hen egg was the only way to isolate and propagate the organism. Since then, a tissue culture system has been available that allows easier… CONTINUE READING

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