Cullin-3 targets cyclin E for ubiquitination and controls S phase in mammalian cells.

@article{Singer1999Cullin3TC,
  title={Cullin-3 targets cyclin E for ubiquitination and controls S phase in mammalian cells.},
  author={Jeffrey D Singer and Mark Gurian-West and Bruce E. Clurman and James M Roberts},
  journal={Genes & development},
  year={1999},
  volume={13 18},
  pages={2375-87}
}
Cyclin E is an unstable protein that is degraded in a ubiquitin- and proteasome- dependent pathway. Two factors stimulate cyclin E ubiquitination in vivo: when it is free of its CDK partner, and when it is phosphorylated on threonine 380. We pursued the first of these pathways by using a two-hybrid screen to identify proteins that could bind only to free cyclin E. This resulted in the isolation of human Cul-3, a member of the cullin family of E3 ubiquitin-protein ligases. We found that Cul-3… CONTINUE READING