Cucurbitacin delta 23-reductase from the fruit of Cucurbita maxima var. Green Hubbard. Physicochemical and fluorescence properties and enzyme-ligand interactions.

H. Dirr; J. C. Schabort; C. Weitz

DOI:10.1042/BJ2330649

Corpus ID: 27484829

Cucurbitacin delta 23-reductase from the fruit of Cucurbita maxima var. Green Hubbard. Physicochemical and fluorescence properties and enzyme-ligand interactions.

@article{Dirr1986CucurbitacinD2,
  title={Cucurbitacin delta 23-reductase from the fruit of Cucurbita maxima var. Green Hubbard. Physicochemical and fluorescence properties and enzyme-ligand interactions.},
  author={H. Dirr and J. C. Schabort and C. Weitz},
  journal={The Biochemical journal},
  year={1986},
  volume={233 3},
  pages={
          649-53
        }
}

H. Dirr, J. C. Schabort, C. Weitz

Published 1986

Chemistry, Medicine

The Biochemical journal

Cucurbitacin delta 23-reductase from Cucurbita maxima var. Green Hubbard fruit displays an apparent Mr of 32,000, a Stokes radius of 263 nm and a diffusion coefficient of 8.93 X 10(-7) cm2 X s-1. The enzyme appears to possess a homogeneous dimeric quaternary structure with a subunit Mr of 15,000. Two tryptophan and fourteen tyrosine residues per dimer were found. Emission spectral properties of the enzyme and fluorescence quenching by iodide indicate the tryptophan residues to be buried within… CONTINUE READING

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