Crystallographic and single-particle analyses of native- and nucleotide-bound forms of the cystic fibrosis transmembrane conductance regulator (CFTR) protein.

@article{Awayn2005CrystallographicAS,
  title={Crystallographic and single-particle analyses of native- and nucleotide-bound forms of the cystic fibrosis transmembrane conductance regulator (CFTR) protein.},
  author={Nuri H Awayn and Mark F. Rosenberg and Alhaji Bukar Kamis and Luba A. Aleksandrov and John R. Riordan and Robert Curtis Ford},
  journal={Biochemical Society transactions},
  year={2005},
  volume={33 Pt 5},
  pages={996-9}
}
Cystic fibrosis, one of the major human inherited diseases, is caused by defects in the CFTR (cystic fibrosis transmembrane conductance regulator), a cell-membrane protein. CFTR acts as a chloride channel which can be opened by ATP. Low-resolution structural studies of purified recombinant human CFTR are described in the present paper. Localization of the C-terminal decahistidine tag in CFTR was achieved by Ni2+-nitriloacetate nanogold labelling, followed by electron microscopy and single… CONTINUE READING

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References

Publications referenced by this paper.
Showing 1-6 of 6 references

J. Biol. Chem

  • J Y Lee, I L Urbatsch, A E Senior, S Wilkens
  • J. Biol. Chem
  • 2002

J. Biol. Chem. J. Struct. Biol

  • A Ferreira-Pereira, S Marco, A Decottignies, J Nader, A Goffeau, J L Rigaud
  • J. Biol. Chem. J. Struct. Biol
  • 1999

J. Mol. Biol

  • M Auer, G A Scarborough, W Kuhlbrandt
  • J. Mol. Biol
  • 1999

Physiol. Rev. Res. Microbiol. Science

  • R A Frizzell
  • Physiol. Rev. Res. Microbiol. Science
  • 1999

Embo J

  • M Cyrklaff, M Auer, W Kuhlbrandt, G A Scarborough
  • EMBO J
  • 1995

Acta Crystallogr. D Biol. Crystallogr

  • G A Scarborough
  • Acta Crystallogr. D Biol. Crystallogr
  • 1994

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