Crystallographic and kinetic evidence of a collision complex formed during halide import in haloalkane dehalogenase.

@article{Pikkemaat1999CrystallographicAK,
  title={Crystallographic and kinetic evidence of a collision complex formed during halide import in haloalkane dehalogenase.},
  author={Mari{\"e}l G. Pikkemaat and I. S. Ridder and Henriette J Rozeboom and Kor H. Kalk and Bauke W Dijkstra and Dick B. Janssen},
  journal={Biochemistry},
  year={1999},
  volume={38 37},
  pages={
          12052-61
        }
}
Haloalkane dehalogenase (DhlA) converts haloalkanes to their corresponding alcohols and halide ions. The rate-limiting step in the reaction of DhlA is the release of the halide ion. The kinetics of halide release have been analyzed by measuring halide binding with stopped-flow fluorescence experiments. At high halide concentrations, halide import occurs predominantly via the rapid formation of a weak initial collision complex, followed by transport of the ion to the active site. To obtain more… CONTINUE READING

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