Crystallization of the middle part of the mitochondrial electron transfer chain: cytochrome bc1-cytochrome c complex.


Complex III (cytochrome bc1 particle; ubiquinol:ferricytochrome c oxidoreductase, EC 1.10.22) was purified from beef heart mitochondria by affinity chromatography. Phospholipids were depleted by washing the particle with detergent while it still was on the affinity column. The particle first was mixed with an excess of cytochrome c in 1.5% cholate (wt/vol… (More)


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