Crystallization of the archaeal transcription termination factor NusA: a significant decrease in twinning under microgravity conditions.

@article{Tanaka2007CrystallizationOT,
  title={Crystallization of the archaeal transcription termination factor NusA: a significant decrease in twinning under microgravity conditions.},
  author={Hiroaki Tanaka and Takashi Umehara and Koji Inaka and Sachiko Takahashi and Rie Shibata and Yoshitaka Bessho and Masaru Sato and Shigeru Sugiyama and Emiko Fusatomi and Takaho Terada and Mikako Shirouzu and Satoshi Sano and Moritoshi Motohara and Tomoyuki Kobayashi and Tetsuo Tanaka and Akiko Kawasaki-ku Tanaka and Shigeyuki Yokoyama},
  journal={Acta crystallographica. Section F, Structural biology and crystallization communications},
  year={2007},
  volume={63 Pt 2},
  pages={
          69-73
        }
}
The transcription termination factor NusA from Aeropyrum pernix was crystallized using a counter-diffusion technique in both terrestrial and microgravity environments. Crystallization under microgravity conditions significantly reduced the twinning content (1.0%) compared with terrestrially grown crystals (18.3%) and improved the maximum resolution from 3.0 to 2.29 A, with similar unit-cell parameters. Based on a comparison of the crystal parameters, the effect of microgravity on protein… CONTINUE READING
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