Crystallization of membrane proteins by vapor diffusion.


X-ray crystallography remains the most robust method to determine protein structure at the atomic level. However, the bottlenecks of protein expression and purification often discourage further study. In this chapter, we address the most common problems encountered at these stages. Based on our experiences in expressing and purifying antimicrobial efflux… (More)
DOI: 10.1016/bs.mie.2014.12.018


Cite this paper

@article{Delmar2015CrystallizationOM, title={Crystallization of membrane proteins by vapor diffusion.}, author={Jared A. Delmar and Jani Reddy Bolla and C. Joanna Su and Edward Yu}, journal={Methods in enzymology}, year={2015}, volume={557}, pages={363-92} }