Crystallization of human plasma apo-retinol-binding protein.

Abstract

Crystals of three forms of human plasma apo-retinol-binding protein have been obtained using the procedure described for the holoprotein. The apoprotein was prepared by a novel method, which uses hydrophobic interaction and immobilized dye chromatography. The three forms were separated by fast protein liquid chromatography. All of the crystals are isomorphous and diffract to 2.5 A resolution. These crystals will be useful for studies of the mechanism of binding of retinol to its carrier using X-ray diffraction techniques.

Statistics

0500201220132014201520162017
Citations per Year

85 Citations

Semantic Scholar estimates that this publication has 85 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Monaco1984CrystallizationOH, title={Crystallization of human plasma apo-retinol-binding protein.}, author={Hugo L. Monaco and Giuseppe Zanotti and Simone Ottonello and Rodolfo Berni}, journal={Journal of molecular biology}, year={1984}, volume={178 2}, pages={477-9} }