Crystallization of human plasma apo-retinol-binding protein.


Crystals of three forms of human plasma apo-retinol-binding protein have been obtained using the procedure described for the holoprotein. The apoprotein was prepared by a novel method, which uses hydrophobic interaction and immobilized dye chromatography. The three forms were separated by fast protein liquid chromatography. All of the crystals are isomorphous and diffract to 2.5 A resolution. These crystals will be useful for studies of the mechanism of binding of retinol to its carrier using X-ray diffraction techniques.


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@article{Monaco1984CrystallizationOH, title={Crystallization of human plasma apo-retinol-binding protein.}, author={Hugo L. Monaco and Giuseppe Zanotti and Simone Ottonello and Rodolfo Berni}, journal={Journal of molecular biology}, year={1984}, volume={178 2}, pages={477-9} }