Crystallization and preliminary crystallographic analysis of a calcineurin B-like protein 1 (CBL1) mutant from Ammopiptanthus mongolicus.

@article{Shang2010CrystallizationAP,
  title={Crystallization and preliminary crystallographic analysis of a calcineurin B-like protein 1 (CBL1) mutant from Ammopiptanthus mongolicus.},
  author={Guijun Shang and Huaixing Cang and Zhijie Liu and Wei Gao and Ruchang Bi},
  journal={Acta crystallographica. Section F, Structural biology and crystallization communications},
  year={2010},
  volume={66 Pt 12},
  pages={1602-5}
}
Calcineurin B-like protein 1 (CBL1) is a calcium sensor in plants. It transmits the calcium signal through the downstream protein CBL-interacting protein kinase (CIPK). CBL1 and CIPK play crucial roles in the response to environmental stresses such as low K+, osmotic shock, high salt, cold and drought. Recombinant CBL1 from Ammopiptanthus mongolicus (AmCBL1) was overexpressed, purified and crystallized. However, the crystal did not diffract well. A mutant prepared using the surface-entropy… CONTINUE READING