Crystallization and preliminary X-ray study of alpha-glucosidase from Geobacillus sp strain HTA-462, one of the deepest sea bacteria.

Abstract

An alpha-glucosidase (EC 3.2.1.20) was purified from Geobacillus sp. strain HTA-462 cells and crystallized using the hanging-drop vapour-diffusion technique. The Geobacillus strain is a thermophilic and high-pressure-resistant bacterium found at the bottom of the Challenger Deep in the Mariana Trench. The crystal was characterized by X-ray diffraction and belongs to space group C2, with unit-cell parameters a = 104.0, b = 91.5, c = 72.9 A, beta = 109.4 degrees. Diffraction data to 2.5 A resolution were collected and processed.

Statistics

0100200300'05'06'07'08'09'10'11'12'13'14'15'16'17
Citations per Year

112 Citations

Semantic Scholar estimates that this publication has 112 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Shirai2003CrystallizationAP, title={Crystallization and preliminary X-ray study of alpha-glucosidase from Geobacillus sp strain HTA-462, one of the deepest sea bacteria.}, author={Tsuyoshi Shirai and Vo Si Hung and Masatake Akita and Yuji Hatada and Susumu Ito and Koki Horikoshi}, journal={Acta crystallographica. Section D, Biological crystallography}, year={2003}, volume={59 Pt 7}, pages={1278-9} }