Crystallization and preliminary X-ray crystallographic studies of Escherichia coli xanthine phosphoribosyltransferase.


Xanthine phosphoribosyltransferase (XPRT; EC from Escherichia coli is a purine salvage enzyme which synthesizes the nucleotides GMP, XMP, and IMP. A mutant C59A, which is more stable than wild-type XPRT while retaining high activity, has been prepared and crystallized to give three different crystal forms (A, B, and C). Form A crystals are… (More)