Crystallization and preliminary X-ray diffraction studies of Hje, a HolliDay junction resolving enzyme from Sulfolobus solfataricus.


HolliDay junction endonuclease (Hje) from Sulfolobus solfataricus is a resolving enzyme involved in cleaving specific sites on either side of recombinant four-way HolliDay junctions. The HJE gene from S. solfataricus was cloned from genomic DNA into the pET19b Escherichia coli expression vector and recombinant protein was expressed to high levels. Hje was… (More)


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