Crystallization and preliminary X-ray diffraction analysis of mevalonate kinase from Methanosarcina mazei.

@article{Zhuang2012CrystallizationAP,
  title={Crystallization and preliminary X-ray diffraction analysis of mevalonate kinase from Methanosarcina mazei.},
  author={Ningning Zhuang and Kyung Hye Seo and Cong Chen and Jia Zhou and Seon-Won Kim and Kon-Ho Lee},
  journal={Acta crystallographica. Section F, Structural biology and crystallization communications},
  year={2012},
  volume={68 Pt 12},
  pages={
          1560-3
        }
}
  • N. Zhuang, K. Seo, Kon-Ho Lee
  • Published 1 December 2012
  • Chemistry
  • Acta crystallographica. Section F, Structural biology and crystallization communications
Mevalonate kinase (MVK), which plays an important role in catalysing the biosynthesis of isoprenoid compounds derived from the mevalonate pathway, transforms mevalonate to 5-phosphomevalonate using ATP as a cofactor. Mevalonate kinase from Methanosarcina mazei (MmMVK) was expressed in Escherichia coli, purified and crystallized for structural analysis. Diffraction-quality crystals of MmMVK were obtained by the vapour-diffusion method using 0.32 M MgCl2, 0.08 M bis-tris pH 5.5, 16%(w/v) PEG 3350… 
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