Crystallization and preliminary X-ray diffraction studies of alpha-toxin from two different strains (NCTC8237 and CER89L43) of Clostridium perfringens.

@article{Basak1998CrystallizationAP,
  title={Crystallization and preliminary X-ray diffraction studies of alpha-toxin from two different strains (NCTC8237 and CER89L43) of Clostridium perfringens.},
  author={Ajit K. Basak and Anne Howells and Julian T. Eaton and D. S. Moss and Claire E. Naylor and Julie Miller and Richard W. Titball},
  journal={Acta crystallographica. Section D, Biological crystallography},
  year={1998},
  volume={54 Pt 6 Pt 2},
  pages={1425-8}
}
The alpha-toxin of Clostridium perfringens is the major virulence determinant for gas gangrene in man. The gene encoding the alpha-toxin has been cloned into E. coli from two strains of the bacterium (NCTC8237 and CER89L43) and subsequently purified to homogeneity. The two strains of alpha-toxin differ by five amino acids, resulting in the toxin from… CONTINUE READING