Crystallization and preliminary X-ray analysis of isomaltase from Saccharomyces cerevisiae.

Abstract

Isomaltase from Saccharomyces cerevisiae is an oligo-1,6-glucosidase that preferentially hydrolyzes isomaltose, with little activity towards isomaltotriose or longer oligosaccharides. An amino-acid sequence analysis of the isomaltase revealed that it belongs to glucoside hydrolase family 13. Recombinant isomaltase was purified and crystallized by the… (More)
DOI: 10.1107/S174430910803114X

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