Crystallization and preliminary X-ray analysis of earthworm fibrinolytic enzyme component A from Eisenia fetida.

Abstract

Earthworm fibrinolytic enzyme component A, a protein which functions both as a direct fibrinolytic enzyme and a plasminogen activator, was purified from the earthworm Eisenia fetida. Diffraction-quality single crystals of the protein were grown by the hanging-drop vapour-diffusion technique with ammonium sulfate as a precipitant. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 40.6, b = 127.5, c = 129.2 A and three molecules per asymmetric unit. The data set reached a resolution of 1.95 A.

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@article{Tang2000CrystallizationAP, title={Crystallization and preliminary X-ray analysis of earthworm fibrinolytic enzyme component A from Eisenia fetida.}, author={Y H Tang and J Zhang and Li Gui and C Y-C Wu and Ruiwen Fan and W R Chang and D Liang}, journal={Acta crystallographica. Section D, Biological crystallography}, year={2000}, volume={56 Pt 12}, pages={1659-61} }