Crystallization and preliminary X-Ray analysis of rotavirus protein VP6.

@article{Petitpas1998CrystallizationAP,
  title={Crystallization and preliminary X-Ray analysis of rotavirus protein VP6.},
  author={Isabelle Petitpas and Jean Lepault and Patrice Vachette and Annie Charpilienne and Magali Mathieu and Evelyne Kohli and Pierre Pothier and Jean Cohen and F{\'e}lix A Rey},
  journal={Journal of virology},
  year={1998},
  volume={72 9},
  pages={7615-9}
}
As a first step to gain insight into the structure of the rotavirus virion at atomic resolution, we report here the expression, purification, and crystallization of recombinant rotavirus protein VP6. This protein has the property of polymerizing in the form of tubular structures in solution which have hindered crystallization thus far. Using a combination of electron microscopy and small-angle X-ray scattering, we found that addition of Ca2+ at concentrations higher than 100 mM results in… CONTINUE READING

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