Crystallization and crystallographic characterization of the iron-sulfur-containing DNA-repair enzyme endonuclease III from Escherichia coli.

@article{Kuo1992CrystallizationAC,
  title={Crystallization and crystallographic characterization of the iron-sulfur-containing DNA-repair enzyme endonuclease III from Escherichia coli.},
  author={Chi Feng Kuo and D. E. Mcree and Richard P. Cunningham and John A. Tainer},
  journal={Journal of molecular biology},
  year={1992},
  volume={227 1},
  pages={
          347-51
        }
}
Endonuclease III from Escherichia coli is an iron-sulfur enzyme possessing both DNA N-glycosylase and apurinic/apyrimidinic lyase activities. It could serve to repair damaged thymine residues in DNA via base excision-repair. We have crystallized endonuclease III by a combination of dialysis and seeding techniques after exploration of a wide variety of precipitants which failed to yield macroscopic crystals. Important features of the optimized crystallization include: the use of 5 to 10… CONTINUE READING
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