Crystalline mammalian L-amino acid oxidase from rat kidney mitochondria.

  title={Crystalline mammalian L-amino acid oxidase from rat kidney mitochondria.},
  author={Minoru Nakano and Thaddeus S. Danowski},
  journal={The Journal of biological chemistry},
  volume={241 9},
L-Amino acid oxidase has been crystallized from extracts of rat kidney mitochondria. Purification involved sonic extraction, ammonium sulfate fractionation, chromatography on diethylaminoethyl cellulose, and gel filtration through Sephadex G-ZOO. The enzyme revealed a single protein band on starch gel electrophoresis and exhibited a single homogeneous peak (a%, W = 10.5 S) in an ultracentrifuge. The enzyme is a flavoprotein in which the prosthetic group appears to be flavin mononucleotide. On… CONTINUE READING