Crystalline beef kidney rhodanese.

@article{Westley1959CrystallineBK,
  title={Crystalline beef kidney rhodanese.},
  author={John W. Westley and James Albert Green},
  journal={The Journal of biological chemistry},
  year={1959},
  volume={234},
  pages={
          2325-6
        }
}
Although rhodanese activity occurs in many tissues (l-3), the enzyme had been obtained in crystalline form from beef liver only (4). Beef kidney rhodanese could not be crystallized by the same procedure (5). To extend the biochemical genetic comparison reported previously (5), however, it was desired to obtain the kidney enzyme in a very high state of purity. This paper reports the purification and crystallization of beef kidney rhodanese by a method which combines an ion exchange fractionation… CONTINUE READING

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SHOWING 1-5 OF 5 REFERENCES

ported by Sijrbo (4) may be related to the differences in the buffers

  • J. WESTLEY
  • J. Biol. Chem.,
  • 1959
Highly Influential
5 Excerpts

line liver rhodanese in this work and the value

  • B. H. SRBO
  • Acta. Chem. &and.,
  • 1953
Highly Influential
3 Excerpts

Chem. Sot., 76, used for the sedimentation runs. A buffer of high ionic strength

  • H. A. SOBER, E. A. PETERSON, J. Am
  • 1954
1 Excerpt

3 The difference between the SZ,,.~ value 3.2 obtained for crystal

  • B H.
  • Acta Chem.. &and.,
  • 1951

since under these conditions all of the enzymatic activity could be recovered from the analytical cell after sedimentation at low tem(1958). peratures. by gest on A uust 27

  • H. A. SOBER, E. A. PETERSON
  • Federation Proc.,
  • 1116
1 Excerpt

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