Crystal structures of the antitermination factor NusB from Thermotoga maritima and implications for RNA binding.

  title={Crystal structures of the antitermination factor NusB from Thermotoga maritima and implications for RNA binding.},
  author={I. Bonin and Rudolf Robelek and H. Benecke and H. Urlaub and A. Bacher and G. Richter and M. Wahl},
  journal={The Biochemical journal},
  volume={383 Pt. 3},
  • I. Bonin, Rudolf Robelek, +4 authors M. Wahl
  • Published 2004
  • Biology, Medicine
  • The Biochemical journal
  • NusB is a prokaryotic transcription factor involved in antitermination processes, during which it interacts with the boxA portion of the mRNA nut site. Previous studies have shown that NusB exhibits an all-helical fold, and that the protein from Escherichia coli forms monomers, while Mycobacterium tuberculosis NusB is a dimer. The functional significance of NusB dimerization is unknown. We have determined five crystal structures of NusB from Thermotoga maritima. In three crystal forms the… CONTINUE READING
    9 Citations
    Structural biophysics of the NusB:NusE antitermination complex.
    • 18
    • PDF
    Structural and functional analysis of the E. coli NusB-S10 transcription antitermination complex.
    • 97
    Nus Factors of Escherichia coli.
    • 12
    Evolutionary comparison of ribosomal operon antitermination function.
    • 23
    • Highly Influenced
    • PDF
    Structure of an archaeal homologue of the bacterial Fmu/RsmB/RrmB rRNA cytosine 5-methyltransferase.
    • 4
    • Highly Influenced
    Subcellular partitioning of transcription factors in Bacillus subtilis.
    • 19
    • PDF
    Structure-based functional inference of hypothetical proteins from Mycoplasma hyopneumoniae
    • 15
    • PDF
    Microbial biochemistry, physiology, and biotechnology of hyperthermophilic Thermotoga species.
    • 99
    • PDF
    Identification of Divergent Protein Domains by Combining HMM-HMM Comparisons and Co-Occurrence Detection
    • 6
    • PDF


    The crystal structure of NusB from Mycobacterium tuberculosis
    • 19
    The structure of the transcriptional antiterminator NusB from Escherichia coli
    • 15
    Ribosomal RNA antitermination in vitro: requirement for Nus factors and one or more unidentified cellular components.
    • 108
    • PDF